Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Lysoganglioside-GM1 from bovine brain >=95%, lyophilized powder | 171483-40-2 | MFCD00677269 | 1MG
Lysoganglioside-GM1 from bovine brain >=95%, lyophilized powder | Purity: >=95% | 171483-40-2 | MFCD00677269 | 1MG
New England Biolabs, Inc. Pyrophosphatase, inorganic (yeast) – 50 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. A variety of metabolic reactions generate inorganic pyrophosphate as a reaction byproduct. Such reactions are rendered irreversible when the pyrophosphate is degraded by pyrophosphatase. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.
New England Biolabs, Inc. PNGase F (Glycerol-free) – 15000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F (Glycerol-free) is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination
- Optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. Endonuclease IV – 1000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase from Clostridium Perfringens (C. welchii) | 9001-67-6 | MFCD00131711 | 5un
Neuraminidase from Clostridium Perfringens (C. welchii) | 9001-67-6 | MFCD00131711 | 5un
Sigma Aldrich Fine Chemicals Biosciences Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) | 9001-40-5 | MFCD00081656 | 1ku
Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae) | Mol Wt: 128 kDa | 9001-40-5 | MFCD00081656 | 1ku
Sigma Aldrich Fine Chemicals Biosciences Thrombin from human plasma | 9002-04-4 | MFCD00082072 | 1KU
Thrombin from human plasma | Mol Wt: 37.4 kDa | 9002-04-4 | MFCD00082072 | 1KU
Sigma Aldrich Fine Chemicals Biosciences Beta-Galactosidase from Escherichia Coli | 9031-11-2 | MFCD00130623 | 1ku
Beta-Galactosidase from Escherichia Coli | 9031-11-2 | MFCD00130623 | 1ku
Sigma Aldrich Fine Chemicals Biosciences Malic Dehydrogenase from porcine heart >=600 units/mg protein (biuret), ammonium sulfate suspension | MFCD00131567 | 5KU
Malic Dehydrogenase from porcine heart >=600 units/mg protein (biuret), ammonium sulfate suspension | MFCD00131567 | 5KU
Sigma Aldrich Fine Chemicals Biosciences Aldehyde Dehydrogenase, potassium-activated from baker's yeast (S. cerevisiae) lyophilized powder, >=2.0 units/mg protein | 9028-88-0 | MFCD00081306 | 100UN
Aldehyde Dehydrogenase, potassium-activated from baker's yeast (S. cerevisiae) lyophilized powder, >=2.0 units/mg protein | 9028-88-0 | MFCD00081306 | 100UN
Sigma Aldrich Fine Chemicals Biosciences Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol) | 9003-99-0 | MFCD00071339 | 10ku
Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol) | Purity: | MW: | 9003-99-0 | MFCD00071339 | 10ku